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Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using HSL Recombinant antibody (84540-1-RR)

    WB analysis of human testis using 84540-1-RR

    Human testis tissue were subjected to SDS PAGE followed by western blot with 84540-1-RR (HSL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HSL Recombinant antibody (84540-1-RR)

    WB analysis using 84540-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84540-1-RR (HSL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using HSL Recombinant antibody (84540-1-RR)

    IHC staining of mouse testis using 84540-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84540-1-RR (LIPE antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using HSL Recombinant antibody (84540-1-RR)

    IHC staining of mouse testis using 84540-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84540-1-RR (LIPE antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using HSL Recombinant antibody (84540-1-RR)

    IHC staining of mouse brown adipose using 84540-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 84540-1-RR (HSL antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using HSL Recombinant antibody (84540-1-RR)

    IHC staining of mouse brown adipose using 84540-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 84540-1-RR (HSL antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84540-1-RR

    Affinity and Kinetic Characterization of 84540-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84540-1-RR against Human HSL were performed. The affinity constant is below 1 pM.

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HSL Recombinant antibody

HSL Recombinant Antibody for WB, IHC, ELISA
Cat No. 84540-1-RR
Clone No.241875B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

LIPE, LHS, Hormone-sensitive lipase, EC:3.1.1.79, EC:3.1.1.23

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman testis tissue, mouse adipose tissue, rat adipose tissue
Positive IHC detected inmouse testis tissue, mouse brown adipose tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84540-1-RR targets HSL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HSL fusion protein Ag11360 相同性解析による交差性が予測される生物種
Full Name lipase, hormone-sensitive
Calculated molecular weight 117 kDa
Observed molecular weight84 kDa
GenBank accession numberBC070041
Gene Symbol HSL
Gene ID (NCBI) 3991
RRIDAB_3672045
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ05469
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hormone sensitive lipase (HSL) belongs to the 'GDXG' lipolytic enzyme family. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. HSL has two isoforms with the molecular mass of 117 kDa and 84 kDa.

Protocols

Product Specific Protocols
WB protocol for HSL antibody 84540-1-RRDownload protocol
IHC protocol for HSL antibody 84540-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols