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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSL Recombinant antibody, PBS Only (Detector)

HSL Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241875B11

Cat no : 84540-1-PBS

Synonyms

LIPE, LHS, Hormone-sensitive lipase, EC:3.1.1.79, EC:3.1.1.23

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using HSL Recombinant antibody (84540-1-RR)

    WB analysis of human testis using 84540-1-RR (same clone as 84540-1-PBS)

    Human testis tissue were subjected to SDS PAGE followed by western blot with 84540-1-RR (HSL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HSL Recombinant antibody (84540-1-RR)

    WB analysis using 84540-1-RR (same clone as 84540-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84540-1-RR (HSL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using HSL Recombinant antibody (84540-1-RR)

    IHC staining of mouse testis using 84540-1-RR (same clone as 84540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84540-1-RR (LIPE antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using HSL Recombinant antibody (84540-1-RR)

    IHC staining of mouse testis using 84540-1-RR (same clone as 84540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84540-1-RR (LIPE antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using HSL Recombinant antibody (84540-1-RR)

    IHC staining of mouse brown adipose using 84540-1-RR (same clone as 84540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 84540-1-RR (HSL antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using HSL Recombinant antibody (84540-1-RR)

    IHC staining of mouse brown adipose using 84540-1-RR (same clone as 84540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 84540-1-RR (HSL antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01374-1

    Cytometric bead array standard curve of MP01374-1

    Cytometric bead array standard curve of MP01374-1, HSL Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84540-3-PBS. Detection antibody: 84540-1-PBS. Standard: Ag11360. Range: 0.625-80 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84540-1-PBS targets HSL as part of a matched antibody pair:

MP01374-1: 84540-3-PBS capture and 84540-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HSL fusion protein Ag11360 相同性解析による交差性が予測される生物種
Full Name lipase, hormone-sensitive
Calculated molecular weight 117 kDa
Observed molecular weight84 kDa
GenBank accession numberBC070041
Gene symbol HSL
Gene ID (NCBI) 3991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Hormone sensitive lipase (HSL) belongs to the 'GDXG' lipolytic enzyme family. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. HSL has two isoforms with the molecular mass of 117 kDa and 84 kDa.