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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated HSP27 Monoclonal antibody

HSP27 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3E7A1

Cat no : CL594-66767

Synonyms

28 kDa heat shock protein, CMT2F, DKFZp586P1322, Heat shock 27 kDa protein, heat shock 27kDa protein 1, Heat shock protein beta 1, HMN2B, HS.76067, HSP 27, Hsp25, HSP27, HSP28, HSPB1, SRP27, Stress responsive protein 27

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated HSP27 Monoclonal antibody (CL594-66767)

    IF Staining of MCF-7 using CL594-66767

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CoraLite®594 HSP27 antibody (CL594-66767, Clone: 3E7A1 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66767 targets HSP27 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSP27 fusion protein Ag27859 相同性解析による交差性が予測される生物種
Full Name heat shock 27kDa protein 1
Calculated molecular weight 19-23 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC012768
Gene symbol HSPB1
Gene ID (NCBI) 3315
RRIDAB_2920032
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPB1, also known as heat shock protein 27 (Hsp27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (21353161).

Protocols

Product Specific Protocols
IF protocol for CL594 HSP27 antibody CL594-66767Download protocol
Standard Protocols
Click here to view our Standard Protocols