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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSP47/SERPINH1 Monoclonal antibody (67863-1-Ig)

    WB analysis using 67863-1-Ig (same clone as 67863-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using HSP47/SERPINH1 Monoclonal antibody (67863-1-Ig)

    WB analysis of NIH/3T3 using 67863-1-Ig (same clone as 67863-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (SERPINH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HSP47/SERPINH1 Monoclonal antibody (67863-1-Ig)

    WB analysis using 67863-1-Ig (same clone as 67863-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using HSP47/SERPINH1 Monoclonal antibody (67863-1-Ig)

    WB analysis of Jurkat using 67863-1-Ig (same clone as 67863-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using HSP47/SERPINH1 Monoclonal antibody (67863-1-Ig)

    WB analysis of LNCaP using 67863-1-Ig (same clone as 67863-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293T cells using HSP47/SERPINH1 Monoclonal antibody (67863-1-Ig)

    WB analysis of HEK-293T using 67863-1-Ig (same clone as 67863-1-PBS)

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (SERPINH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using HSP47/SERPINH1 Monoclonal antibody (67863-1-Ig)

    IHC staining of human renal cell carcinoma using 67863-1-Ig (same clone as 67863-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using HSP47/SERPINH1 Monoclonal antibody (67863-1-Ig)

    IHC staining of human renal cell carcinoma using 67863-1-Ig (same clone as 67863-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSP47/SERPINH1 Monoclonal antibody (67863-1-Ig)

    IF Staining of HeLa using 67863-1-Ig (same clone as 67863-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HSP47/SERPINH1 Monoclonal antibody (67863-1-Ig)

    IF Staining of HepG2 using 67863-1-Ig (same clone as 67863-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67863-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50781-1

    Cytometric bead array standard curve of MP50781-1

    Cytometric bead array standard curve of MP50781-1, HSP47/SERPINH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67863-2-PBS. Detection antibody: 67863-1-PBS. Standard:Ag30999. Range: 0.781-100 ng/mL.

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HSP47/SERPINH1 Monoclonal antibody, PBS Only (Detector)

HSP47/SERPINH1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67863-1-PBS
Clone No.2A8F6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

HSP47, SERPINH1, CBP2, CBP1, AsTP3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67863-1-PBS targets HSP47/SERPINH1 as part of a matched antibody pair:

MP50781-1: 67863-2-PBS capture and 67863-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSP47/SERPINH1 fusion protein Ag30999 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Calculated molecular weight 46 kDa
Observed molecular weight46 kDa
GenBank accession numberBC014623
Gene Symbol HSP47/SERPINH1
Gene ID (NCBI) 871
RRIDAB_2918621
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50454
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.