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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSP47 Monoclonal antibody (67863-1-Ig)

    WB analysis using 67863-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using HSP47 Monoclonal antibody (67863-1-Ig)

    WB analysis of NIH/3T3 using 67863-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (SERPINH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HSP47 Monoclonal antibody (67863-1-Ig)

    WB analysis using 67863-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using HSP47 Monoclonal antibody (67863-1-Ig)

    WB analysis of LNCaP using 67863-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using HSP47 Monoclonal antibody (67863-1-Ig)

    WB analysis of Jurkat using 67863-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (HSP47 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using HSP47 Monoclonal antibody (67863-1-Ig)

    WB analysis of HEK-293T using 67863-1-Ig

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 67863-1-Ig (SERPINH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using HSP47 Monoclonal antibody (67863-1-Ig)

    IHC staining of human renal cell carcinoma using 67863-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using HSP47 Monoclonal antibody (67863-1-Ig)

    IHC staining of human renal cell carcinoma using 67863-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67863-1-Ig (HSP47 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSP47/SERPINH1 Monoclonal antibody (67863-1-Ig)

    IF Staining of HeLa using 67863-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HSP47/SERPINH1 Monoclonal antibody (67863-1-Ig)

    IF Staining of HepG2 using 67863-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP47 antibody (67863-1-Ig, Clone: 2A8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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HSP47/SERPINH1 Monoclonal antibody

HSP47/SERPINH1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67863-1-Ig
Clone No.2A8F6
Publications(3)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

HSP47, SERPINH1, CBP2, CBP1, AsTP3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  150 μL, 1000 ug/ml
20 μL, 1000 ug/ml
150 μL, 1000 ug/ml

¥67,000/ 150 μL

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, HEK-293T cells, NIH/3T3 cells, LNCaP cells, HeLa cells, Jurkat cells, HEK-293 cells, HSC-T6 cells, 4T1 cells
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67863-1-Ig targets HSP47/SERPINH1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSP47/SERPINH1 fusion protein Ag30999 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Calculated molecular weight 46 kDa
Observed molecular weight46 kDa
GenBank accession numberBC014623
Gene Symbol HSP47/SERPINH1
Gene ID (NCBI) 871
RRIDAB_2918621
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50454
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HSP47/SERPINH1 antibody 67863-1-IgDownload protocol
IHC protocol for HSP47/SERPINH1 antibody 67863-1-IgDownload protocol
IF protocol for HSP47/SERPINH1 antibody 67863-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF,CoIP

iScience

SERPINH1 modulates apoptosis by inhibiting P62 ubiquitination degradation to promote bone metastasis of prostate cancer

Authors - Chen Tang
  • KD Validated
View Article
ratWB

J Proteome Res

Data-Independent Acquisition Proteomics and N-Terminomics Methods Reveal Alterations in Mitochondrial Function and Metabolism in Ischemic-Reperfused Hearts

Authors - Bridgette Hartley
View Article
humanWB,IP,CoIP

Elife

Hsp47 promotes biogenesis of multi-subunit neuroreceptors in the endoplasmic reticulum

Authors - Ya-Juan Wang
View Article