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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated HSP47/SERPINH1 Monoclonal antibody

HSP47/SERPINH1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A8F6

Cat no : CL488-67863

Synonyms

HSP47, SERPINH1, CBP2, CBP1, AsTP3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated HSP47 Monoclonal ant (CL488-67863)

    IF Staining of HepG2 using CL488-67863

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HSP47 antibody (CL488-67863, Clone: 2A8F6 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated HSP47 Monoclonal ant (CL488-67863)

    IF Staining of HeLa using CL488-67863

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 HSP47 antibody (CL488-67863, Clone: 2A8F6 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated HSP47 Monoclonal ant (CL488-67863)

    IF Staining of HeLa using CL488-67863

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 HSP47 antibody (CL488-67863, Clone: 2A8F6 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated HSP47 Monoclonal ant (CL488-67863)

    IF Staining of HepG2 using CL488-67863

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HSP47 antibody (CL488-67863, Clone: 2A8F6 ) at dilution of 1:200, CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated HSP47 Monoclonal ant (CL488-67863)

    FC experiment of HepG2 using CL488-67863

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human HSP47 (CL488-67863, Clone:2A8F6) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67863 targets HSP47/SERPINH1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSP47/SERPINH1 fusion protein Ag30999 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Calculated molecular weight 46 kDa
Observed molecular weight46 kDa
GenBank accession numberBC014623
Gene symbol HSP47/SERPINH1
Gene ID (NCBI) 871
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HSP47/SERPINH1 antibody CL488-67863Download protocol
Standard Protocols
Click here to view our Standard Protocols