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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSP47/SERPINH1 Recombinant antibody

HSP47/SERPINH1 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240752F6

Cat no : 83689-3-RR

Synonyms

HSP47, SERPINH1, CBP2, CBP1, AsTP3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSP47/SERPINH1 Recombinant antibody (83689-3-RR)

    WB analysis using 83689-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83689-3-RR (HSP47/SERPINH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using HSP47/SERPINH1 Recombinant antibody (83689-3-RR)

    IHC staining of human ovarian cancer using 83689-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83689-3-RR (HSP47/SERPINH1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HepG2 cells using HSP47/SERPINH1 Recombinant antibody (83689-3-RR)

    FC experiment of HepG2 using 83689-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HSP47/SERPINH1 Recombinant antibody (83689-3-RR, Clone:240752F6) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83689-3-RR

    Affinity and Kinetic Characterization of 83689-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83689-3-RR against Human HSP47/SERPINH1 were performed. The affinity constant is 12.9 nM.

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Tested Applications

Positive WB detected inHeLa cells, K-562 cells, HT-1080 cells, SK-N-SH cells, NIH/3T3 cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83689-3-RR is HSP47/SERPINH1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Calculated molecular weight46kDa
Observed molecular weight 46 kDa
GenBank accession numberNM_001235.5
Gene symbol HSP47/SERPINH1
Gene ID (NCBI) 871
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSP47 is also named as Serpin H1, colligen, CBP1, CBP2, HSP47, SERPINH2 and belongs to the serpin family. This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum. It binds specifically to collagen and can be involved as a chaperone in the biosynthetic pathway of collagen. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. HSP47 has a calculated molecular weight of 46 kDa.

Protocols

Product Specific Protocols
WB protocol for HSP47/SERPINH1 antibody 83689-3-RRDownload protocol
IHC protocol for HSP47/SERPINH1 antibody 83689-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols