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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSP47/SERPINH1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human, mouse

Detection: human, mouse

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.23-15 ng/mL

Cat No : MP00677-2

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00677-2, Human HSP47/SERPINH1 Recombinant Matched Antibody Pair - PBS only. 83689-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1201. 83689-3-PBS was HRP conjugated as the detection antibody. Range: 0.23-15 ng/mL

    Sandwich ELISA standard curve of MP00677-2

    Sandwich ELISA standard curve of MP00677-2, Human HSP47/SERPINH1 Recombinant Matched Antibody Pair - PBS only. 83689-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1201. 83689-3-PBS was HRP conjugated as the detection antibody. Range: 0.23-15 ng/mL

  • HepG2, Hela and MCF-7 cells were cultured in DMEM supplemented with 10% fetal bonive serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Aliquots of the cell culture supernates were removed. Cells were lysed and assayed for human HSP47/SERPINH1. Human HSP47/SERPINH1 in HepG2 cell lysate measured 17.9 ng/mL. Human HSP47/SERPINH1 in Hela cell lysate measured 56.9 ng/mL. Human HSP47/SERPINH1 in MCF-7 cell lysate measured 35.1 ng/mL.

    Sample test of MP00677-2

    HepG2, Hela and MCF-7 cells were cultured in DMEM supplemented with 10% fetal bonive serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Aliquots of the cell culture supernates were removed. Cells were lysed and assayed for human HSP47/SERPINH1. Human HSP47/SERPINH1 in HepG2 cell lysate measured 17.9 ng/mL. Human HSP47/SERPINH1 in Hela cell lysate measured 56.9 ng/mL. Human HSP47/SERPINH1 in MCF-7 cell lysate measured 35.1 ng/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00677-2 targets HSP47/SERPINH1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.23-15 ng/mL (Sandwich ELISA);

Capture antibody: HSP47/SERPINH1 Recombinant antibody, PBS Only (Capture) 83689-4-PBS (240752F2). 100 μg. Concentration 1 mgl/ml.

Detection antibody: HSP47/SERPINH1 Recombinant antibody, PBS Only (Detector) 83689-3-PBS (240752F6). 100 μg. Concentration 1 mgl/ml.

Alternative HSP47/SERPINH1 matched antibody pairs: MP00677-1

Matched pair protein standard: Eg1201

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83689-4-PBS
Tested Reactivity human, mouse
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Synonyms HSP47, SERPINH1, CBP2, CBP1, AsTP3
GenBank accession numberNM_001235.5
Gene symbol HSP47/SERPINH1
UniprotP50454
Gene ID (NCBI) 871
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purfication
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83689-3-PBS
Tested Reactivity human, mouse
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Synonyms HSP47, SERPINH1, CBP2, CBP1, AsTP3
GenBank accession numberNM_001235.5
Gene symbol HSP47/SERPINH1
UniprotP50454
Gene ID (NCBI) 871
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purfication
Storage Buffer PBS only
Storage Conditions Store at -80°C.