CoraLite® Plus 488-conjugated HSP70 Monoclonal antibody
HSP70 Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, yeast
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4E7E5
Cat no : CL488-66183
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HEK-293 cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66183 targets HSP70 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, yeast samples.
Tested Reactivity | human, mouse, rat, yeast |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | HSP70 fusion protein Ag19111 相同性解析による交差性が予測される生物種 |
Full Name | heat shock 70kDa protein 1A |
Calculated molecular weight | 70 kDa |
Observed molecular weight | 65-70 kDa |
GenBank accession number | BC009322 |
Gene symbol | HSP70 |
Gene ID (NCBI) | 3303 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker. This antibody well recognized the endogenous HSP70 protein in multiple cell lines. (21373891)
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 HSP70 antibody CL488-66183 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |