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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated HSP70 Polyclonal antibody (CL594-10995)

    IF Staining of HeLa using CL594-10995

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 HSP70 antibody (CL594-10995) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated HSP70 Polyclonal antibody (CL594-10995)

    FC experiment of HeLa using CL594-10995

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human HSP70 (CL594-10995) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated HSP70 Polyclonal antibody

HSP70 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-10995

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

HSPA1A, Heat shock 70 kDa protein 1A, heat shock 70kDa protein 1A, Heat shock protein family A member 1A, HSP70.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10995 targets HSP70 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSP70 fusion protein Ag1446 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 1A
Calculated molecular weight 70 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC009322
Gene Symbol HSP70
Gene ID (NCBI) 3303
RRIDAB_3084639
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP0DMV8
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

  1. What is Hsp70/HSP1A?

    HSP1A is a member of the Hsp70 (heat shock protein 70) proteins that act as molecular chaperones ensuring correct protein folding and preventing protein aggregation. Hsp70 protein production is greatly induced by various stress stimuli, including high temperature and toxins. Its expression is often elevated in various cancers.

  2. FAQs for Hsp70

    a. I cannot detect Hsp70 by western blotting

    HSP70s are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Try to always include cell lysate from cells subjected to stress conditions as a positive control.

    b. What loading control can I use for cellular stress experiments with Hsp70?

    Choosing a loading control antibody is an important step in western blotting experimental setup. We highly recommend using more than one loading control while developing new cellular stress assays to ensure that a given treatment does not alter expression of house-keeping genes. Hsp70 has a molecular size of 70 kDa, so we recommend using GAPDH (36 kDa), actin (42 kDa), or tubulin (50-55 kDa). More information on our control antibodies can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.

Protocols

Product Specific Protocols
IF protocol for CL594 HSP70 antibody CL594-10995Download protocol
Standard Protocols
Click here to view our Standard Protocols