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  • Featured Product
  • KD/KO Validated

HSP90 Polyclonal antibody

HSP90 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 13171-1-AP

Synonyms

HSP90AA1, HSP90A, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90 alpha, Heat shock protein family C member 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSP90 Polyclonal antibody (13171-1-AP)

    WB analysis using 13171-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13171-1-AP (HSP90 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HSP90 Polyclonal antibody (13171-1-AP)

    WB analysis using 13171-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13171-1-AP (HSP90 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using HSP90 Polyclonal antibody (13171-1-AP)

    IP experiment of K-562 using 13171-1-AP

    IP result of anti-HSP90 (IP:13171-1-AP, 3ug; Detection:13171-1-AP 1:500) with K-562 cells lysate 2000ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using HSP90 Polyclonal antibody (13171-1-AP)

    IP experiment of HeLa using 13171-1-AP

    IP result of anti-HSP90 (IP:13171-1-AP, 3ug; Detection:13171-1-AP 1:500) with HeLa cells lysate 4400ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using HSP90 Polyclonal antibody (13171-1-AP)

    IHC staining of mouse testis using 13171-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13171-1-AP (HSP90 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using HSP90 Polyclonal antibody (13171-1-AP)

    IHC staining of mouse testis using 13171-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13171-1-AP (HSP90 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using HSP90 Polyclonal antibody (13171-1-AP)

    IHC staining of human colon using 13171-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13171-1-AP (HSP90 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HSP90 Polyclonal antibody (13171-1-AP)

    IF Staining of HepG2 using 13171-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP90 antibody (13171-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HSP90 Polyclonal antibody (13171-1-AP)

    IF Staining of HepG2 using 13171-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP90 antibody (13171-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using HSP90 Polyclonal antibody (13171-1-AP)

    FC experiment of HeLa using 13171-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug HSP90 Polyclonal antibody (13171-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, PC-12 cells, C6 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inK-562 cells, HeLa cells
Positive IHC detected inmouse testis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1600-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13171-1-AP targets HSP90 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, chicken, bovine, sheep, fish, carp
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSP90 fusion protein Ag3826 相同性解析による交差性が予測される生物種
Full Name heat shock protein 90kDa alpha (cytosolic), class A member 1
Calculated molecular weight 853 aa, 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC023006
Gene symbol HSP90
Gene ID (NCBI) 3320
RRIDAB_2120924
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.

Protocols

Product Specific Protocols
WB protocol for HSP90 antibody 13171-1-APDownload protocol
IHC protocol for HSP90 antibody 13171-1-APDownload protocol
IF protocol for HSP90 antibody 13171-1-APDownload protocol
IP protocol for HSP90 antibody 13171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.

Authors - Lingyun Long
View Article
mouseWB

Cell Metab

IgG is an aging factor that drives adipose tissue fibrosis and metabolic decline

Authors - Lexiang Yu
View Article
CoIP

Nat Cell Biol

LIMIT is an immunogenic lncRNA in cancer immunity and immunotherapy.

Authors - Gaopeng Li
View Article
mouseWB

Bioact Mater

Genetically engineered M2-like macrophage-derived exosomes for P. gingivalis-suppressed cementum regeneration: From mechanism to therapy

Authors - Xin Huang
View Article
mouseWB

Nat Commun

The CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8+ T cells

Authors - Xiaofeng Liao
View Article
mouse,ratWB

Nat Commun

Protective effects of Pt-N-C single-atom nanozymes against myocardial ischemia-reperfusion injury

Authors - Tianbao Ye
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

S (Verified Customer) (08-14-2024)

excellent

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: hek
HSP90 Antibody Western Blot validation (1000 dilution) in hek (Cat no:13171-1-AP)
FH

Satyendra (Verified Customer) (07-06-2022)

Works very nicely with CEF, DF-1 and VeroE6 cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: VeroE6 cells
FH

Guorong (Verified Customer) (03-22-2022)

Excellent performance with a specific band of expected size

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2500
  • Cell Tissue Type: ARPE-19
HSP90 Antibody Western Blot validation (1:2500 dilution) in ARPE-19 (Cat no:13171-1-AP)
FH

Ekaterini (Verified Customer) (02-16-2022)

The best antibody to use for loading control. Always clear and it is not affected by drug treatments.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: ALL cell lines
FH

Xiaoping (Verified Customer) (10-22-2018)

This antibody works well in iPS, iMns or MEF. The signal is pretty strong which is better than the one we used before and the background is very low.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human neuron and mouse fibroblast samples
HSP90 Antibody Western Blot, validation (1:1000 dilution) in Human neuron and mouse fibroblast samples (Cat no:13171-1-AP)