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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated HSP90 Polyclonal antibody

HSP90 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13171

Synonyms

HSP90AA1, HSP90A, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90 alpha, Heat shock protein family C member 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated HSP90 Polyclonal ant (CL488-13171)

    IF Staining of HepG2 using CL488-13171

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 HSP90 antibody (CL488-13171) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated HSP90 Polyclonal ant (CL488-13171)

    FC experiment of HeLa using CL488-13171

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Hsp90 Polyclonal Antibody (CL488-13171)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13171 targets HSP90 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSP90 fusion protein Ag3826 相同性解析による交差性が予測される生物種
Full Name heat shock protein 90kDa alpha (cytosolic), class A member 1
Calculated molecular weight 853 aa, 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC023006
Gene symbol HSP90
Gene ID (NCBI) 3320
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HSP90 antibody CL488-13171Download protocol
FC protocol for CL Plus 488 HSP90 antibody CL488-13171Download protocol
Standard Protocols
Click here to view our Standard Protocols