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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSP90AB1 Recombinant antibody (80301-1-RR)

    WB analysis using 80301-1-RR (same clone as 80301-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80301-1-RR (HSP90AB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of zebrafish tissue using HSP90AB1 Recombinant antibody (80301-1-RR)

    WB analysis of zebrafish using 80301-1-RR (same clone as 80301-1-PBS)

    zebrafish tissue were subjected to SDS PAGE followed by western blot with 80301-1-RR (HSP90AB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using HSP90AB1 Recombinant antibody (80301-1-RR)

    WB analysis of HEK-293 using 80301-1-RR (same clone as 80301-1-PBS)

    WB result of HSP90AB1 antibody (80301-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells. This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HSP90AB1 Recombinant antibody (80301-1-RR)

    IHC staining of human breast cancer using 80301-1-RR (same clone as 80301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80301-1-RR (HSP90AB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSP90AB1 Recombinant antibody (80301-1-RR)

    IF Staining of HeLa using 80301-1-RR (same clone as 80301-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HSP90AB1 antibody (80301-1-RR, Clone: 5P14 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80301-1-RR

    Affinity and Kinetic Characterization of 80301-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80301-1-RR against Human HSP90AB1 were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

HSP90AB1 Recombinant antibody, PBS Only

HSP90AB1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 80301-1-PBS
Clone No.5P14

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, zebrafish

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HSPC2, HSP90B, Heat shock protein HSP 90-beta, Heat shock protein HSP 90 beta, Heat shock protein family C member 3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80301-1-PBS targets HSP90AB1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, zebrafish samples.

Tested Reactivity Human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HSP90AB1 fusion protein Ag1947 相同性解析による交差性が予測される生物種
Full Name heat shock protein 90kDa alpha (cytosolic), class B member 1
Calculated molecular weight 723 aa, 83 kDa
GenBank accession numberBC014485
Gene Symbol HSP90AB1
Gene ID (NCBI) 3326
RRIDAB_2918885
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08238
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers.