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  • Featured Product
  • KD/KO Validated

HSP90AB1 Recombinant antibody

HSP90AB1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, zebrafish

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5P14

Cat no : 80301-1-RR

Synonyms

HSPC2, HSP90B, Heat shock protein HSP 90-beta, Heat shock protein HSP 90 beta, Heat shock protein family C member 3

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using HSP90AB1 Recombinant antibody (80301-1-RR)

    WB analysis of HEK-293 using 80301-1-RR

    WB result of HSP90AB1 antibody (80301-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using HSP90AB1 Recombinant antibody (80301-1-RR)

    WB analysis using 80301-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80301-1-RR (HSP90AB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of zebrafish tissue using HSP90AB1 Recombinant antibody (80301-1-RR)

    WB analysis of zebrafish using 80301-1-RR

    zebrafish tissue were subjected to SDS PAGE followed by western blot with 80301-1-RR (HSP90AB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HSP90AB1 Recombinant antibody (80301-1-RR)

    IHC staining of human breast cancer using 80301-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80301-1-RR (HSP90AB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSP90AB1 Recombinant antibody (80301-1-RR)

    IF Staining of HeLa using 80301-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HSP90AB1 antibody (80301-1-RR, Clone: 5P14 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 80301-1-RR

    Affinity and Kinetic Characterization of 80301-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80301-1-RR against Human HSP90AB1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inLNCaP cells, zebrafish tissue, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, Neuro-2a cells, HSC-T6 cells, PC-12 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80301-1-RR targets HSP90AB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, zebrafish samples.

Tested Reactivity Human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HSP90AB1 fusion protein Ag1947 相同性解析による交差性が予測される生物種
Full Name heat shock protein 90kDa alpha (cytosolic), class B member 1
Calculated molecular weight 723 aa, 83 kDa
GenBank accession numberBC014485
Gene symbol HSP90AB1
Gene ID (NCBI) 3326
RRIDAB_2918885
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers.

Protocols

Product Specific Protocols
WB protocol for HSP90AB1 antibody 80301-1-RRDownload protocol
IHC protocol for HSP90AB1 antibody 80301-1-RRDownload protocol
IF protocol for HSP90AB1 antibody 80301-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols