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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-HSP27 (Ser15) Recombinant antibody, PBS Only

Phospho-HSP27 (Ser15) Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D4

Cat no : 83332-3-PBS

Synonyms

HSP27, HSPB1, Hsp25, HMN2B, Estrogen-regulated 24 kDa protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-HSP27 (Ser15) Recombinant antibody (83332-3-RR)

    WB analysis using 83332-3-RR (same clone as 83332-3-PBS)

    Non-treated HeLa cells, and anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 83332-3-RR (Phospho-HSP27 (Ser15) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin(66009-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 83332-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-HSP27 (Ser15) Recombinant antibody (83332-3-RR)

    IF Staining of HeLa using 83332-3-RR (same clone as 83332-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Anisomycin treated HeLa cells using Phospho-HSP27 (Ser15) antibody (83332-3-RR, Clone: 2D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83332-3-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83332-3-PBS targets Phospho-HSP27 (Ser15) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name heat shock 27kDa protein 1
Calculated molecular weight 19-23 kDa
Observed molecular weight27 kDa
GenBank accession numberBC012768
Gene symbol HSPB1
Gene ID (NCBI) 3315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HSPB1, also named as HSP27, belongs to the mammalian small HSP family widely expressed in cells including the cytoplasm and nucleus. It has been reported that HSPB1 plays a key role in stress resistance, actin organization and numerous biological processes. HSPB1 can be phosphorylated in different stimulation such as cytokines, growth factors and peptide hormones on the site of Ser15, Ser78 and Ser82 by MAPKAP2 kinase. The phosphorylation of HSPB1 leads to a decrease in the size of HSPB1 oligomers. HSPB1 is associated with amyotrophic lateral sclerosis (ALS) (PMID: 8542580, 11960785, 10383393, 32231288)