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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-HSP27 (Ser15) Recombinant antibody

Phospho-HSP27 (Ser15) Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D4

Cat no : 83332-3-RR

Synonyms

Hsp27 (phospho S15), HSP27 (Ser15), Phospho HSP27 (Ser15), phospho-Hsp27, Phospho-HSP27 Ser15

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Phospho-HSP27 (Ser15) Recombinant antibody (83332-3-RR)

    WB analysis of HeLa using 83332-3-RR

    Non-treated HeLa cells, and anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 83332-3-RR (Phospho-HSP27 (Ser15) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin(66009-1-Ig) antibody as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-HSP27 (Ser15) Recombinant antibody (83332-3-RR)

    IF Staining of HeLa using 83332-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed Anisomycin treated HeLa cells using Phospho-HSP27 (Ser15) antibody (83332-3-RR, Clone: 2D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inAnisomycin treated HeLa cells
Positive IF/ICC detected inAnisomycin treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83332-3-RR targets Phospho-HSP27 (Ser15) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name heat shock 27kDa protein 1
Calculated molecular weight 19-23 kDa
Observed molecular weight27 kDa
GenBank accession numberBC012768
Gene symbol HSPB1
Gene ID (NCBI) 3315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPB1, also named as HSP27, belongs to the mammalian small HSP family widely expressed in cells including the cytoplasm and nucleus. It has been reported that HSPB1 plays a key role in stress resistance, actin organization and numerous biological processes. HSPB1 can be phosphorylated in different stimulation such as cytokines, growth factors and peptide hormones on the site of Ser15, Ser78 and Ser82 by MAPKAP2 kinase. The phosphorylation of HSPB1 leads to a decrease in the size of HSPB1 oligomers. HSPB1 is associated with amyotrophic lateral sclerosis (ALS) (PMID: 8542580, 11960785, 10383393, 32231288)

Protocols

Product Specific Protocols
WB protocol for Phospho-HSP27 (Ser15) antibody 83332-3-RRDownload protocol
IF protocol for Phospho-HSP27 (Ser15) antibody 83332-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols