CoraLite® Plus 488-conjugated HSPB3 Monoclonal antibody
HSPB3 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
Human, rabbit, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2A10B5
Cat no : CL488-67890
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67890 targets HSPB3 in FC (Intra) applications and shows reactivity with Human, rabbit, mouse, rat samples.
| Tested Reactivity | Human, rabbit, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | HSPB3 fusion protein Ag26940 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock 27kDa protein 3 |
| Calculated molecular weight | 150 aa, 17 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC126272 |
| Gene symbol | HSPB3 |
| Gene ID (NCBI) | 8988 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Heat shock protein family B (small) member 3 (HSPB3) is the third member of the sHSP family in human and is mainly expressed in skeletal and smooth muscles, and can associate with HSPB2, which is another sHSP prominently expressed in cardiac and skeletal muscles. HSPB3 is involved in several cellular functions such as cell signaling, differentiation, and apoptosis, and mutations of HSPB3 are associated with neuromuscular diseases, such as axonal Charcot‐Marie‐Tooth disease (PMID: 31709619) (PMID: 32093037). The molecular weight of HSPB3 is about 17 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 HSPB3 antibody CL488-67890 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
