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HSP60 Polyclonal antibody

Name: HSP60 Polyclonal antibody
Brand: Proteintech
SKU: 15282-1-AP

HSP60 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

145

Review Product

Cat no : 15282-1-AP

Synonyms

HSPD1, Heat shock protein 60, GROEL, EC:5.6.1.7, CPN60

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 15282-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15282-1-AP (HSP60 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15282-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15282-1-AP (HSP60 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 15282-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15282-1-AP (HSP60 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 15282-1-AP
IP result of anti-HSP60 (IP:15282-1-AP, 4ug; Detection:15282-1-AP 1:10000) with HEK-293 cells lysate 1800 ug.
IHC staining of human lung cancer using 15282-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15282-1-AP (HSP60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15282-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15282-1-AP (HSP60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15282-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15282-1-AP (HSP60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15282-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15282-1-AP (HSP60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15282-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP60 antibody (15282-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).
FC experiment of HepG2 using 15282-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human HSP60 (15282-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

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Cat No. 15282-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HepG2 cells, NIH/3T3 cells, HeLa, mouse heart, rat heart
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 120 publications below
IHC	See 8 publications below
IF	See 32 publications below
IP	See 3 publications below
ELISA	See 1 publications below
CoIP	See 1 publications below

Product Information

15282-1-AP targets HSP60 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, zebrafish, goat, yeast, fish, duck
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HSP60 fusion protein Ag7401 相同性解析による交差性が予測される生物種
Full Name	heat shock 60kDa protein 1 (chaperonin)
Calculated molecular weight	61 kDa
Observed molecular weight	60 kDa
GenBank accession number	BC003030
Gene symbol	HSP60
Gene ID (NCBI)	3329
RRID	AB_2121440
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)

Protocols

Product Specific Protocols
WB protocol for HSP60 antibody 15282-1-AP	Download protocol
IHC protocol for HSP60 antibody 15282-1-AP	Download protocol
IF protocol for HSP60 antibody 15282-1-AP	Download protocol
IP protocol for HSP60 antibody 15282-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation. Authors - Xiaorong Zhang View Article
mouse	WB	Nucleic Acids Res RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis Authors - Chao Liu View Article
mouse	WB	J Hazard Mater Long-term effect of PBDE-99 prenatal exposure on spermatogenic injuries via the dysregulation of autophagy Authors - Jun Wu View Article
human	WB	Sci Adv Pathogenic mitochondrial DNA mutations inhibit melanoma metastasis Authors - Spencer D Shelton View Article
human	WB	Nat Commun Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response. Authors - Wenjun Wang View Article
mouse	WB,IF	Nat Commun Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells. Authors - Gustavo Della-Flora Nunes View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSP60 Polyclonal antibody

HSP60 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 15282-1-AP

WB analysis of HepG2 using 15282-1-AP

WB analysis of NIH/3T3 using 15282-1-AP

IP experiment of HEK-293 using 15282-1-AP

IHC staining of human lung cancer using 15282-1-AP

IHC staining of human lung cancer using 15282-1-AP

IHC staining of human lung cancer using 15282-1-AP

IHC staining of human lung cancer using 15282-1-AP

IF Staining of HeLa using 15282-1-AP

FC experiment of HepG2 using 15282-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.

RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis

Long-term effect of PBDE-99 prenatal exposure on spermatogenic injuries via the dysregulation of autophagy

Pathogenic mitochondrial DNA mutations inhibit melanoma metastasis

Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.

Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells.

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