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- KD/KO Validated
HSP60 Polyclonal antibody
HSP60 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Conjugate
Unconjugated
136
Cat no : 15282-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, NIH/3T3 cells, HeLa, mouse heart, rat heart |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 111 publications below |
| IHC | See 7 publications below |
| IF | See 29 publications below |
| IP | See 1 publications below |
| ELISA | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
15282-1-AP targets HSP60 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, zebrafish, goat, yeast, fish, duck |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HSP60 fusion protein Ag7401 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock 60kDa protein 1 (chaperonin) |
| Calculated molecular weight | 61 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC003030 |
| Gene symbol | HSP60 |
| Gene ID (NCBI) | 3329 |
| RRID | AB_2121440 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HSP60 antibody 15282-1-AP | Download protocol |
| IHC protocol for HSP60 antibody 15282-1-AP | Download protocol |
| IF protocol for HSP60 antibody 15282-1-AP | Download protocol |
| IP protocol for HSP60 antibody 15282-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis | ||
J Hazard Mater Long-term effect of PBDE-99 prenatal exposure on spermatogenic injuries via the dysregulation of autophagy | ||
Nat Commun Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response. | ||
Nat Commun Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells. | ||
Nat Commun EGFL9 promotes breast cancer metastasis by inducing cMET activation and metabolic reprogramming. |
