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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 647-conjugated HSP60 Polyclonal ant (CL647-15282)

    IF Staining of HepG2 using CL647-15282

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 HSP60 antibody (CL647-15282) at dilution of 1:200, CL488-phalloidin (green).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 647-conjugated HSP60 Polyclonal ant (CL647-15282)

    FC experiment of HepG2 using CL647-15282

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human HSP60 (CL647-15282) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated HSP60 Polyclonal antibody

HSP60 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-15282

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

HSPD1, 60 kDa chaperonin, Chaperonin 60, CPN60, EC:5.6.1.7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-15282 targets HSP60 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSP60 fusion protein Ag7401 相同性解析による交差性が予測される生物種
Full Name heat shock 60kDa protein 1 (chaperonin)
Calculated molecular weight 61 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC003030
Gene Symbol HSP60
Gene ID (NCBI) 3329
RRIDAB_2934914
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10809
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 HSP60 antibody CL647-15282Download protocol
FC protocol for CL Plus 647 HSP60 antibody CL647-15282Download protocol
Standard Protocols
Click here to view our Standard Protocols