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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using HVCN1 Monoclonal antibody (66449-1-Ig)

    WB analysis of Raji using 66449-1-Ig (same clone as 66449-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66449-1-Ig (HVCN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HVCN1 Monoclonal antibody (66449-1-Ig)

    WB analysis of HeLa using 66449-1-Ig (same clone as 66449-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66449-1-Ig (HVCN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HVCN1 Monoclonal antibody (66449-1-Ig)

    IHC staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66449-1-Ig (HVCN1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HVCN1 Monoclonal antibody (66449-1-Ig)

    IHC staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66449-1-Ig (HVCN1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using HVCN1 Monoclonal antibody (66449-1-Ig)

    IF Staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HVCN1 antibody (66449-1-Ig, Clone: 1E4C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using HVCN1 Monoclonal antibody (66449-1-Ig)

    IF Staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HVCN1 antibody (66449-1-Ig, Clone: 1E4C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

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HVCN1 Monoclonal antibody, PBS Only

HVCN1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66449-1-PBS
Clone No.1E4C4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

HV1, HVCN1, VSOP

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66449-1-PBS targets HVCN1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HVCN1 fusion protein Ag5350 相同性解析による交差性が予測される生物種
Full Name hydrogen voltage-gated channel 1
Calculated molecular weight 273 aa, 32 kDa
Observed molecular weight 28-35 kDa, 40 kDa, 60 kDa
GenBank accession numberBC032672
Gene Symbol HVCN1
Gene ID (NCBI) 84329
RRIDAB_2881818
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96D96
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

HVCN1, also named as VSOP and HV1, Belongs to the hydrogen channel family. HVCN1 mediates the voltage-dependent proton permeability of excitable membranes. It forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, HVCN1 is accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis. HVCN1, the voltage-sensitive proton channel, is present in human sperm and is an important regulator of the functional maturation of sperm. HVCN1 has four isoforms with MW 28-32 kDa or 40 kDa (modification). It has a dimer form with MW 60 kDa.