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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using HVCN1 Monoclonal antibody (66449-1-Ig)

    WB analysis of Raji using 66449-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66449-1-Ig (HVCN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HVCN1 Monoclonal antibody (66449-1-Ig)

    WB analysis of HeLa using 66449-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66449-1-Ig (HVCN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HVCN1 Monoclonal antibody (66449-1-Ig)

    IHC staining of human tonsillitis using 66449-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66449-1-Ig (HVCN1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HVCN1 Monoclonal antibody (66449-1-Ig)

    IHC staining of human tonsillitis using 66449-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66449-1-Ig (HVCN1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using HVCN1 Monoclonal antibody (66449-1-Ig)

    IF Staining of human tonsillitis using 66449-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HVCN1 antibody (66449-1-Ig, Clone: 1E4C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using HVCN1 Monoclonal antibody (66449-1-Ig)

    IF Staining of human tonsillitis using 66449-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HVCN1 antibody (66449-1-Ig, Clone: 1E4C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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HVCN1 Monoclonal antibody

HVCN1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66449-1-Ig
Clone No.1E4C4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

HV1, HVCN1, VSOP

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells, HeLa cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66449-1-Ig targets HVCN1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HVCN1 fusion protein Ag5350 相同性解析による交差性が予測される生物種
Full Name hydrogen voltage-gated channel 1
Calculated molecular weight 273 aa, 32 kDa
Observed molecular weight 28-35 kDa, 40 kDa, 60 kDa
GenBank accession numberBC032672
Gene Symbol HVCN1
Gene ID (NCBI) 84329
RRIDAB_2881818
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96D96
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HVCN1, also named as VSOP and HV1, Belongs to the hydrogen channel family. HVCN1 mediates the voltage-dependent proton permeability of excitable membranes. It forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, HVCN1 is accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis. HVCN1, the voltage-sensitive proton channel, is present in human sperm and is an important regulator of the functional maturation of sperm. HVCN1 has four isoforms with MW 28-32 kDa or 40 kDa (modification). It has a dimer form with MW 60 kDa.

Protocols

Product Specific Protocols
WB protocol for HVCN1 antibody 66449-1-IgDownload protocol
IHC protocol for HVCN1 antibody 66449-1-IgDownload protocol
IF protocol for HVCN1 antibody 66449-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols