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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated HVCN1 Monoclonal antibody (CL594-66449)

    IF Staining of human tonsillitis using CL594-66449

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 HVCN1 antibody (CL594-66449, Clone: 1E4C4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated HVCN1 Monoclonal antibody (CL594-66449)

    IF Staining of human tonsillitis using CL594-66449

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 HVCN1 antibody (CL594-66449, Clone: 1E4C4 ) at dilution of 1:200.

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CoraLite®594-conjugated HVCN1 Monoclonal antibody

HVCN1 Monoclonal Antibody for IF-P
Cat No. CL594-66449
Clone No.1E4C4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF-P

Synonyms

HV1, HVCN1, VSOP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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Tested Applications

Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66449 targets HVCN1 in IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HVCN1 fusion protein Ag5350 相同性解析による交差性が予測される生物種
Full Name hydrogen voltage-gated channel 1
Calculated molecular weight 273 aa, 32 kDa
Observed molecular weight 28-35 kDa, 40 kDa, 60 kDa
GenBank accession numberBC032672
Gene Symbol HVCN1
Gene ID (NCBI) 84329
RRIDAB_2919994
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96D96
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HVCN1, also named as VSOP and HV1, Belongs to the hydrogen channel family. HVCN1 mediates the voltage-dependent proton permeability of excitable membranes. It forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, HVCN1 is accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis. HVCN1, the voltage-sensitive proton channel, is present in human sperm and is an important regulator of the functional maturation of sperm. HVCN1 has four isoforms with MW 28-32 kDa or 40 kDa (modification). It has a dimer form with MW 60 kDa.

Protocols

Product Specific Protocols
IF protocol for CL594 HVCN1 antibody CL594-66449Download protocol
Standard Protocols
Click here to view our Standard Protocols