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  • Featured Product
  • KD/KO Validated

HYAL2 Polyclonal antibody

HYAL2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51148-1-AP

Synonyms

Hyal 2, HYAL2, Hyaluronidase 2, hyaluronoglucosaminidase 2, LuCa 2, LUCA2, Lung carcinoma protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using HYAL2 Polyclonal antibody (51148-1-AP)

    WB analysis of mouse heart using 51148-1-AP

    mouse heart tissue and mouse liver tissue were subjected to SDS PAGE followed by western blot with 51148-1-AP (HYAL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using HYAL2 Polyclonal antibody (51148-1-AP)

    WB analysis of human heart using 51148-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 51148-1-AP (HYAL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human lung tissue using HYAL2 Polyclonal antibody (51148-1-AP)

    WB analysis of human lung using 51148-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 51148-1-AP (HYAL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HYAL2 Polyclonal antibody (51148-1-AP)

    IHC staining of human lung cancer using 51148-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51148-1-AP (HYAL2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HYAL2 Polyclonal antibody (51148-1-AP)

    IHC staining of human lung cancer using 51148-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51148-1-AP (HYAL2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HYAL2 Polyclonal antibody (51148-1-AP)

    IF Staining of HeLa using 51148-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 51148-1-AP (HYAL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue, human lung tissue, mouse liver tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51148-1-AP targets HYAL2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HYAL2 fusion protein Ag0582 相同性解析による交差性が予測される生物種
Full Name hyaluronoglucosaminidase 2
Calculated molecular weight 54 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC000692
Gene symbol HYAL2
Gene ID (NCBI) 8692
RRIDAB_2123391
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hyaluronic acid (HA) is a glycosaminoglycan that is believed to have numerous important biologic functions, including modulation of cell proliferation, migration, and differentiation, as well as the regulation of extracellular water and protein homeostasis. It is also an integral structural component of cartilage and other tissues and acts as a lubricant in joints. Hyaluronidases are a family of enzymes that catalyse the degradation of HA. In humans, there are five functional hyaluronidases: HYAL1, HYAL2, HYAL3, HYAL4 and HYAL5 (also known as SPAM1 or PH-20); plus a pseudogene, HYAL6 (also known as HYALP1). HYAL2 is present in many tissues except the adult brain (PMID: 11731268). HYAL2 can be exposed to cell surfaces that bind to the plasma membrane via GPI anchors (PMID: 11731268).

Protocols

Product Specific Protocols
WB protocol for HYAL2 antibody 51148-1-APDownload protocol
IHC protocol for HYAL2 antibody 51148-1-APDownload protocol
IF protocol for HYAL2 antibody 51148-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Dev Cell

High-Molecular-Weight Hyaluronan Is a Hippo Pathway Ligand Directing Cell Density-Dependent Growth Inhibition via PAR1b.

Authors - Takuya Ooki
  • KD Validated
View Article