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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HYAL3 Polyclonal antibody

HYAL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19625-1-AP

Synonyms

Hyal 3, HYAL3, Hyaluronidase 3, hyaluronoglucosaminidase 3, LUCA 3, LUCA14, LUCA3, Lung carcinoma protein 3, Minna14

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using HYAL3 Polyclonal antibody (19625-1-AP)

    WB analysis of human brain using 19625-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19625-1-AP (HYAL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using HYAL3 Polyclonal antibody (19625-1-AP)

    WB analysis of HepG2 using 19625-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19625-1-AP (HYAL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using HYAL3 Polyclonal antibody (19625-1-AP)

    IHC staining of human testis using 19625-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19625-1-AP (HYAL3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using HYAL3 Polyclonal antibody (19625-1-AP)

    IHC staining of human testis using 19625-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19625-1-AP (HYAL3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using HYAL3 Polyclonal antibody (19625-1-AP)

    IHC staining of human testis using 19625-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19625-1-AP (HYAL3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using HYAL3 Polyclonal antibody (19625-1-AP)

    IHC staining of human testis using 19625-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19625-1-AP (HYAL3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HYAL3 Polyclonal antibody (19625-1-AP)

    IHC staining of human prostate cancer using 19625-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 19625-1-AP (HYAL3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HYAL3 Polyclonal antibody (19625-1-AP)

    IHC staining of human prostate cancer using 19625-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 19625-1-AP (HYAL3 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inhuman brain tissue, HepG2 cells
Positive IHC detected inhuman testis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 19625-1-AP is HYAL3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HYAL3 fusion protein Ag8611 相同性解析による交差性が予測される生物種
Full Name hyaluronoglucosaminidase 3
Calculated molecular weight417aa,47 kDa; 463aa,52 kDa
Observed molecular weight 47 kDa, 55 kDa
GenBank accession numberBC005896
Gene symbol HYAL3
Gene ID (NCBI) 8372
RRIDAB_10666437
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HYAL3 antibody 19625-1-APDownload protocol
IHC protocol for HYAL3 antibody 19625-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Gastroenterol Hepatol

The suppressive role of HYAL1 and HYAL2 in the metastasis of colorectal cancer.

Authors - Zhiming Jin
View Article