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ChromoTek Halo-Trap Magnetic Particles M-270

The ChromoTek Halo-Trap Magnetic Particles M-270 consists of an anti-Halo-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. Halo-Trap Magnetic Particles M-270 is used to immunoprecipitate Halo-tag proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. in the presence or absence of a covalently bound ligand. The interaction between Halo-Trap and the Halo-tag protein is reversible.

Specificity

Halo-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Particles M-270

Type

Nanobody

Cat no : otd

Synonyms

Halo Trap Magnetic Particles M-270, Halo-Trap Magnetic Particles M-270, Halo-Trap, Halo, Halo tag, otd

Validation Data Gallery

  • Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. Western blot was probed with Halo antibody [28A8] (28a8) and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] (sms1AF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

    Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. Western blot was probed with Halo antibody [28A8] (28a8) and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] (sms1AF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

  • Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

    Halo-Trap Magnetic Particles M-270 was used for immunoprecipitation of Halo-tag protein from HEK293T cell lysate and elution with 2x SDS-sample buffer. Coomassie blue staining shows elution of enriched Halo-tag protein. L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

Product Information

The ChromoTek Halo-Trap Magnetic Particles M-270 consists of an anti-Halo-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. Halo-Trap Magnetic Particles M-270 is used to immunoprecipitate Halo-tag proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. in the presence or absence of a covalently bound ligand. The interaction between Halo-Trap and the Halo-tag protein is reversible.

DescriptionImmunoprecipitation of Halo-tagged proteins and their interacting factors with anti-Halo Nanobody conjugated to magnetic particles. Halo-Trap Magnetic Particles M-270 is recommended when very large proteins/complexes are investigated and magnetic separation is needed for IP.

• Fast, reliable & efficient one-step immunoprecipitation

• Halo-Trap immunoprecipitates Halo-fusion proteins even when already covalently bound to Halo-ligands, i.e. after labelling etc.

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetHalo-tag (modified variant of the bacterial haloalkane dehalogenase enzyme from Rhodococcus rhodochrous) in the absence or presence of covalently bound chloralkane-based ligands.
Binding capacity1.25 μg of recombinant Halo-tag per 25 μL bead slurry
ConjugateMagnetic Particles M-270, size: 2.8 µm
high throughput-compatible
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility2 M urea, 2 M NaCl, 10 mM DTT,  2 % Nonidet P40 Substitute, 2 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 2 nM
Compatibility with mass spectrometryThe Halo-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2894835
Storage BufferPBS with 0.09% sodium azide
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Rick (Verified Customer) (07-22-2022)

It worked well, just few troubleshooting. used succesfully on cancer line and HEK cells

  • Applications: Protein purification
  • Cell Tissue Type: HEK & cancer line