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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 647-conjugated Heavy chain of Rabbi (CL647-66467)

    IF Staining of HeLa using CL647-66467

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using the CoraLite® Plus 647-conjugated version of this antibody, CL647-66467 (Heavy chain of Rabbit IgG antibody), at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated Heavy chain of Rabbi (CL647-66467)

    FC experiment of HeLa using CL647-66467

    1X10^6 HeLa cells were intracellularly stained with Beta Catenin Polyclonal antibody (51067-2-AP) and 0.4 ug CoraLite® Plus 647 Anti-Rabbit Heavy chain of Rabbit IgG (CL647-66467, Clone:1C9A5) (red), or Control Antibody and 0.4 ug CoraLite® Plus 647 Anti-Rabbit Heavy chain of Rabbit IgG (CL647-66467, Clone:1C9A5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 647-conjugated Heavy chain of Rabbit IgG Monoclonal antibody

Heavy chain of Rabbit IgG Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-66467
Clone No.1C9A5

Host / Isotype

Mouse / IgG1

Reactivity

rabbit

Applications

IF/ICC, FC (Intra)

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66467 targets Heavy chain of Rabbit IgG in IF/ICC, FC (Intra) applications and shows reactivity with rabbit samples.

Tested Reactivity rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Protein 相同性解析による交差性が予測される生物種
Full Name Heavy chain of Rabbit IgG
Gene Symbol
Gene ID (NCBI)
RRIDAB_2883736
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDN/A
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody specifically recognizes the heavy chain of Rabbit IgG.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Heavy chain of Rabbit IgG antibody CL647-66467Download protocol
Standard Protocols
Click here to view our Standard Protocols