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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Hexokinase 2 Monoclonal antibody

Hexokinase 2 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A11C3

Cat no : CL594-66974

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite®594-conjugated Hexokinase 2 Monoclonal an (CL594-66974)

    WB analysis using CL594-66974

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-66974 (Hexokinase 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated Hexokinase 2 Monoclonal an (CL594-66974)

    FC experiment of HepG2 using CL594-66974

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Coralite®594 Hexokinase 2 Monoclonal Antibody (CL594-66974, Clone:2A11C3)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66974 targets Hexokinase 2 in WB applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Hexokinase 2 fusion protein Ag16895 相同性解析による交差性が予測される生物種
Full Name hexokinase 2
Calculated molecular weight 102 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC021116
Gene symbol Hexokinase 2
Gene ID (NCBI) 3099
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236)

Protocols

Product Specific Protocols
WB protocol for CL594 Hexokinase 2 antibody CL594-66974Download protocol
Standard Protocols
Click here to view our Standard Protocols