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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-Histone H2A.X (Ser139) Recombinant antibody, PBS Only

Phospho-Histone H2A.X (Ser139) Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5N19

Cat no : 83307-2-PBS

Synonyms

H2A histone family, member X, H2A.X, H2A/X, H2AFX, H2AX, Histone H2A.x

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Histone H2A.X (Ser139) Recombinant antibod (83307-2-RR)

    WB analysis using 83307-2-RR (same clone as 83307-2-PBS)

    Non-treated Jurkat cells, and staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 83307-2-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83307-2-PBS targets Phospho-Histone H2A.X (Ser139) in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name H2A histone family, member X
Calculated molecular weight 15 kDa
GenBank accession numberBC013416
Gene symbol H2AFX
Gene ID (NCBI) 3014
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.