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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-Histone H2A.X (Ser139) Recombinant antibody

Phospho-Histone H2A.X (Ser139) Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5N19

Cat no : 83307-2-RR

Synonyms

H2AFX, Histone H2A, H2AX, H2A.X, H2A histone family, member X

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-Histone H2A.X (Ser139) Recombinant antibod (83307-2-RR)

    WB analysis using 83307-2-RR

    Non-treated Jurkat cells, and staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control.

  • Immunohistochemistry (IHC) staining of Jurkat cells using Phospho-Histone H2A.X (Ser139) Recombinant antibod (83307-2-RR)

    IHC staining of Jurkat using 83307-2-RR

    Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 83307-2-RR (Phospho-Histone H2A.X (Ser139) antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-Histone H2A.X (Ser139) Recombinant antibod (83307-2-RR)

    IF Staining of HeLa using 83307-2-RR

    Immunofluorescent analysis of (4% PFA) fixed UV treated HeLa cells using Phospho-Histone H2A.X (Ser139) antibody (83307-2-RR, Clone: 5N19 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inStaurosporine treated Jurkat cells
Positive IHC detected inJurkat cells
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inUV treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

83307-2-RR targets Phospho-Histone H2A.X (Ser139) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name H2A histone family, member X
Calculated molecular weight 15 kDa
Observed molecular weight15 kDa
GenBank accession numberBC013416
Gene symbol Histone H2A.X
Gene ID (NCBI) 3014
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The histone variant H2AX is a major component of the DNA damage response (DDR), especially functioning in amplifying DNA damage signals. In response to DNA double-strand breaks (DSBs), H2AX is instantaneously phosphorylated at Ser139 (a form called cH2AX) by the kinases ATM and ATR. The phosphorylation of H2AX at Ser139, resulting in the formation of gamma-H2AX puncta in the nuclei, is an early event in the cellular response to DNA damage. Therefore, phospho-Histone H2A. X (Ser139) is also known as γH2AX. The phosphorylation site of H2AX, Ser139, has also been described as Ser140 in other literature, and they recognize the same amino acid site. (PMID: 22908299, PMID: 30106130, PMID:22941631)

Protocols

Product Specific Protocols
WB protocol for Phospho-Histone H2A.X (Ser139) antibody 83307-2-RRDownload protocol
IHC protocol for Phospho-Histone H2A.X (Ser139) antibody 83307-2-RRDownload protocol
IF protocol for Phospho-Histone H2A.X (Ser139) antibody 83307-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Transl Oncol

PiR-hsa-23533 promotes malignancy in head and neck squamous cell carcinoma via USP7

Authors - Hanlin Hu
View Article