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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Histone H2B Polyclonal antibody, PBS Only

Histone H2B Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27740-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using Histone H2B Polyclonal antibody (27740-1-AP)

    WB analysis of HEK-293T using 27740-1-AP (same clone as 27740-1-PBS)

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Histone H2B Polyclonal antibody (27740-1-AP)

    WB analysis using 27740-1-AP (same clone as 27740-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Histone H2B Polyclonal antibody (27740-1-AP)

    WB analysis of HEK-293 using 27740-1-AP (same clone as 27740-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Histone H2B Polyclonal antibody (27740-1-AP)

    IF Staining of HEK-293 using 27740-1-AP (same clone as 27740-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Histone H2B antibody (27740-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27740-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27740-1-PBS targets Histone H2B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Histone H2B fusion protein Ag26901 相同性解析による交差性が予測される生物種
Full Name histone cluster 3, H2bb
Observed molecular weight15-20 kDa
GenBank accession numberBC100855
Gene symbol Histone H2B
Gene ID (NCBI) 128312
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST3H2BB is a replication-dependent histone that is a member of the histone H2B family.