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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Histone H2B Monoclonal antibody (68393-1-Ig)

    WB analysis using 68393-1-Ig (same clone as 68393-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68393-1-Ig (Histone H2B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Histone H2B Monoclonal antibody (68393-1-Ig)

    IHC staining of human lung cancer using 68393-1-Ig (same clone as 68393-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68393-1-Ig (Histone H2B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Histone H2B Monoclonal antibody (68393-1-Ig)

    IHC staining of human lung cancer using 68393-1-Ig (same clone as 68393-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68393-1-Ig (Histone H2B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Histone H2B Monoclonal antibody (68393-1-Ig)

    IF Staining of HeLa using 68393-1-Ig (same clone as 68393-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Histone H2B antibody (68393-1-Ig, Clone: 1E12G3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Histone H2B Monoclonal antibody (68393-1-Ig)

    IF Staining of HeLa using 68393-1-Ig (same clone as 68393-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H2B antibody (68393-1-Ig, Clone: 1E12G3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Histone H2B Monoclonal antibody (68393-1-Ig)

    FC experiment of HeLa using 68393-1-Ig (same clone as 68393-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Histone H2B (68393-1-Ig, Clone:1E12G3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

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Histone H2B Monoclonal antibody, PBS Only

Histone H2B Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68393-1-PBS
Clone No.1E12G3

Host / Isotype

Mouse / IgG1

Reactivity

zebrafish, chicken, rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

HIST2H2BE, H2BC21, H2B/q, H2B.1, H2B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68393-1-PBS targets Histone H2B in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with zebrafish, chicken, rat, mouse, human samples.

Tested Reactivity zebrafish, chicken, rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 2, H2be
Calculated molecular weight 14 kDa
Observed molecular weight 14-17 kDa
GenBank accession numberBC005827
Gene Symbol Histone H2B
Gene ID (NCBI) 8349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16778
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).