Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, chicken cerebellum tissue, zebrafish tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
68393-1-Ig targets Histone H2B in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, chicken, zebrafish samples.
| Tested Reactivity | human, mouse, rat, chicken, zebrafish |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | histone cluster 2, H2be |
| Calculated molecular weight | 14 kDa |
| Observed molecular weight | 14-17 kDa |
| GenBank accession number | BC005827 |
| Gene Symbol | Histone H2B |
| Gene ID (NCBI) | 8349 |
| RRID | AB_3085110 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q16778 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Histone H2B antibody 68393-1-Ig | Download protocol |
| IHC protocol for Histone H2B antibody 68393-1-Ig | Download protocol |
| IF protocol for Histone H2B antibody 68393-1-Ig | Download protocol |
| FC protocol for Histone H2B antibody 68393-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Clin Epigenetics Herbal medicine formula Huazhuo Tiaozhi granule ameliorates dyslipidaemia via regulating histone lactylation and miR-155-5p biogenesis |