Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-68393 targets Histone H2B in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Zebrafish, Chicken samples.
| Tested Reactivity | Human, Mouse, Rat, Zebrafish, Chicken |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | histone cluster 2, H2be |
| Calculated molecular weight | 14 kDa |
| Observed molecular weight | 14-17 kDa |
| GenBank accession number | BC005827 |
| Gene Symbol | Histone H2B |
| Gene ID (NCBI) | 8349 |
| RRID | AB_3084480 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q16778 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Histone H2B antibody CL488-68393 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |