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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Histone H2B Monoclonal antibody

Histone H2B Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Zebrafish, Chicken

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E12G3

Cat no : CL488-68393

Synonyms

GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone cluster 2, H2be, Histone H2B, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Histone H2B Monoclon (CL488-68393)

    IF Staining of HeLa using CL488-68393

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Histone H2B antibody (CL488-68393, Clone: 1E12G3 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68393 targets Histone H2B in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Zebrafish, Chicken samples.

Tested Reactivity Human, Mouse, Rat, Zebrafish, Chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 2, H2be
Calculated molecular weight 14 kDa
Observed molecular weight 14-17 kDa
GenBank accession numberBC005827
Gene symbol Histone H2B
Gene ID (NCBI) 8349
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Histone H2B antibody CL488-68393Download protocol
Standard Protocols
Click here to view our Standard Protocols