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  • KD/KO Validated

HuR/ELAVL1 Polyclonal antibody

HuR/ELAVL1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 11910-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, CLIP

ELAVL1, Hua, Hu antigen R, ELAV-like protein 1, ELAVL 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11910-1-AP targets HuR/ELAVL1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, CLIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HuR/ELAVL1 fusion protein Ag2249 相同性解析による交差性が予測される生物種
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
Calculated molecular weight 326 aa, 36 kDa
Observed molecular weight 30-37 kDa
GenBank accession numberBC003376
Gene Symbol HuR/ELAVL1
Gene ID (NCBI) 1994
RRIDAB_11182183
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15717
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

Protocols

Product Specific Protocols
WB protocol for HuR/ELAVL1 antibody 11910-1-APDownload protocol
IHC protocol for HuR/ELAVL1 antibody 11910-1-APDownload protocol
IF protocol for HuR/ELAVL1 antibody 11910-1-APDownload protocol
IP protocol for HuR/ELAVL1 antibody 11910-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cancer

SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers

Authors - Lei Shi
humanWB

Nat Protoc

Global analysis of RNA-binding protein dynamics by comparative and enhanced RNA interactome capture.

Authors - Joel I Perez-Perri
humanIF

Acta Neuropathol

Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis.

Authors - Sandra Diaz-Garcia
mouseWB

Mol Cell

Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal.

Authors - Jing Wen
humanWB

Autophagy

m6A reader YTHDC1 modulates autophagy by targeting SQSTM1 in diabetic skin

Authors - Diefei Liang
  • KD Validated
humanWB

Nat Commun

Discovery of RNA-binding proteins and characterization of their dynamic responses by enhanced RNA interactome capture.

Authors - Joel I Perez-Perri
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