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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated HuR/ELAVL1 Polyclonal antibody

HuR/ELAVL1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-11910

Synonyms

ELAVL1, Hua, Hu antigen R, ELAV-like protein 1, ELAVL 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated HuR/ELAVL1 Polyclonal anti (CL594-11910)

    IF Staining of MCF-7 using CL594-11910

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 HuR/ELAVL1 antibody (CL594-11910) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite®594-conjugated HuR/ELAVL1 Polyclonal anti (CL594-11910)

    FC experiment of MCF-7 using CL594-11910

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human HuR/ELAVL1 (CL594-11910) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-11910 targets HuR/ELAVL1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HuR/ELAVL1 fusion protein Ag2249 相同性解析による交差性が予測される生物種
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
Calculated molecular weight 326 aa, 36 kDa
Observed molecular weight 30-37 kDa
GenBank accession numberBC003376
Gene symbol HuR/ELAVL1
Gene ID (NCBI) 1994
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

Protocols

Product Specific Protocols
IF protocol for CL594 HuR/ELAVL1 antibody CL594-11910Download protocol
FC protocol for CL594 HuR/ELAVL1 antibody CL594-11910Download protocol
Standard Protocols
Click here to view our Standard Protocols