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Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using Human IgA Monoclonal antibody (60099-1-Ig)

    WB analysis of human saliva using 60099-1-Ig (same clone as 60099-1-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 60099-1-Ig (Human IgA Antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human plasma using Human IgA Monoclonal antibody (60099-1-Ig)

    WB analysis of human plasma using 60099-1-Ig (same clone as 60099-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 60099-1-Ig (Human IgA Antibody) at dilution of 1:64000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Human IgA Monoclonal antibody (60099-1-Ig)

    IHC staining of human tonsillitis using 60099-1-Ig (same clone as 60099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60099-1-Ig (Human IgA antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Human IgA Monoclonal antibody (60099-1-Ig)

    IHC staining of human tonsillitis using 60099-1-Ig (same clone as 60099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60099-1-Ig (Human IgA antibody) at dilution of 1:320000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Human IgA Monoclonal antibody (60099-1-Ig)

    IHC staining of human tonsillitis using 60099-1-Ig (same clone as 60099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60099-1-Ig (Human IgA antibody) at dilution of 1:320000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using Human IgA Monoclonal antibody (60099-1-Ig)

    IF Staining of human tonsillitis using 60099-1-Ig (same clone as 60099-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgA antibody (60099-1-Ig, Clone: 3A5A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.

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Human IgA Monoclonal antibody, PBS Only

Human IgA Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 60099-1-PBS
Clone No.3A5A5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Human IgA Heavy Chain, IGHA1, 3A5A5, IGHA 1, Igh2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60099-1-PBS targets Human IgA in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Human IgA fusion protein Ag7417 相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant alpha 1
Calculated molecular weight 496 aa, 53 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC016369
Gene Symbol Human IgA Heavy Chain
Gene ID (NCBI) 3493
RRIDAB_1557448
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).