Validation Data Gallery
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Tested Applications
| Positive WB detected in | human saliva tissue, human saliva, human plasma |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:160000-1:640000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
60099-1-Ig targets Human IgA in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Human IgA fusion protein Ag7417 相同性解析による交差性が予測される生物種 |
| Full Name | immunoglobulin heavy constant alpha 1 |
| Calculated molecular weight | 496 aa, 53 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | BC016369 |
| Gene Symbol | Human IgA Heavy Chain |
| Gene ID (NCBI) | 3493 |
| RRID | AB_1557448 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Human IgA antibody 60099-1-Ig | Download protocol |
| IHC protocol for Human IgA antibody 60099-1-Ig | Download protocol |
| IF protocol for Human IgA antibody 60099-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology A milk-based self-assemble rotavirus VP6-ferritin nanoparticle vaccine elicited protection against the viral infection. | ||
Eur J Pharmacol Inhibition of excessive autophagy alleviates renal injury and inflammation in a rat model of immunoglobulin A nephropathy | ||
Hepatol Commun Spatial mapping of the HCC landscape identifies unique intratumoral perivascular-immune neighborhoods |