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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Biotin-conjugated IgA Monoclonal antibody

Human IgA Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC

Conjugate

Biotin

CloneNo.

3A5A5

Cat no : Biotin-60099

Synonyms

FLJ14473, FLJ35065, FLJ35500, FLJ36402, FLJ39698, FLJ40001, FLJ41548, FLJ41552, FLJ41789, FLJ43248, FLJ43594, FLJ44293, FLJ46028, FLJ46621, FLJ46724, FLJ46811, FLJ46824, FLJ90170, IgA1, IGHA1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Biotin-conjugated Human IgA Monoclonal antibody (Biotin-60099)

    IHC staining of human tonsillitis using Biotin-60099

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-60099 (Human IgA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-60099 targets Human IgA in IHC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Human IgA fusion protein Ag7417 相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant alpha 1
Calculated molecular weight 496 aa, 53 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC016369
Gene symbol Human IgA Heavy Chain
Gene ID (NCBI) 3493
RRIDAB_2918945
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).

Protocols

Product Specific Protocols
IHC protocol for Biotin Human IgA antibody Biotin-60099Download protocol
Standard Protocols
Click here to view our Standard Protocols