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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HRP-conjugated IgA Monoclonal antibody

Human IgA Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB

Conjugate

HRP

CloneNo.

3A5A5

Cat no : HRP-60099

Synonyms

FLJ14473, FLJ35065, FLJ35500, FLJ36402, FLJ39698, FLJ40001, FLJ41548, FLJ41552, FLJ41789, FLJ43248, FLJ43594, FLJ44293, FLJ46028, FLJ46621, FLJ46724, FLJ46811, FLJ46824, FLJ90170, Human IgA, IgA1, IGHA1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HRP-conjugated Human IgA Monoclonal antibody (HRP-60099)

    WB analysis using HRP-60099

    50 ug, 25 ug, 12.5 ug human saliva were subjected to SDS PAGE followed by western blot with HRP-60099 (Human IgA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using HRP-conjugated Human IgA Monoclonal antibody (HRP-60099)

    WB analysis of human placenta using HRP-60099

    human placenta tissue were subjected to SDS PAGE followed by western blot with HRP-60099 (Human IgA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using HRP-conjugated Human IgA Monoclonal antibody (HRP-60099)

    WB analysis of human plasma using HRP-60099

    human plasma were subjected to SDS PAGE followed by western blot with HRP-60099 (Human IgA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inhuman saliva, human placenta tissue, human plasma

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

HRP-60099 targets Human IgA in WB applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Human IgA fusion protein Ag7417 相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant alpha 1
Calculated molecular weight 496 aa, 53 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC016369
Gene symbol Human IgA Heavy Chain
Gene ID (NCBI) 3493
RRIDAB_2883830
Conjugate HRP
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).

Protocols

Product Specific Protocols
WB protocol for HRP Human IgA antibody HRP-60099Download protocol
Standard Protocols
Click here to view our Standard Protocols