Validation Data Gallery

Filter: AllWBIHCIF-P

WB analysis of human plasma using 10284-1-AP
human plasma were subjected to SDS PAGE followed by western blot with 10284-1-AP (Human IgG antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 10284-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10284-1-AP (Human IgG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 10284-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10284-1-AP (Human IgG antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human tonsillitis using 10284-1-AP
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgG antibody (10284-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Human IgG Polyclonal antibody

Human IgG Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 10284-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, IP, CoIP, ChIP, ELISA

Synonyms

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Biotin CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (20)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human plasma tissue
Positive IHC detected in	human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human tonsillitis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:20000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IP	See 1 publications below
CoIP	See 3 publications below
ChIP	See 5 publications below

Product Information

10284-1-AP targets Human IgG in WB, IHC, IF-P, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein 相同性解析による交差性が予測される生物種
Full Name	Human IgG
Calculated molecular weight	155 kDa
Observed molecular weight	25 kDa, 50 kDa
Gene Symbol
Gene ID (NCBI)
RRID	AB_2877729
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

This rabbit poly clonal antibody recognizes the human IgG.

Protocols

Product Specific Protocols
WB protocol for Human IgG antibody 10284-1-AP	Download protocol
IHC protocol for Human IgG antibody 10284-1-AP	Download protocol
IF protocol for Human IgG antibody 10284-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Sci Adv Therapeutic strategies targeting uPAR potentiate anti-PD-1 efficacy in diffuse-type gastric cancer. Authors - Long Qin View Article
		Int Immunopharmacol LncRNA-H19 induces hepatic stellate cell activation via upregulating alcohol dehydrogenase III-mediated retinoic acid signals. Authors - Zhi-Min Wang View Article
	WB	Arch Biochem Biophys Fructose-derived glycation and immune function: Effects on antigen binding in human IgG and lymphocytes Authors - Feryal Akay View Article
		Front Bioeng Biotechnol Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes. Authors - Rui-Rui Guo View Article
		Biosensors (Basel) Compact Surface Plasmon Resonance IgG Sensor Based on H-Shaped Optical Fiber. Authors - Yijian Huang View Article
	CoIP	Int J Oral Sci FAM64A promotes HNSCC tumorigenesis by mediating transcriptional autoregulation of FOXM1. Authors - Xinyuan Zhao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human plasma using 10284-1-AP

IHC staining of human tonsillitis using 10284-1-AP

IHC staining of human tonsillitis using 10284-1-AP

IF Staining of human tonsillitis using 10284-1-AP

Human IgG Polyclonal antibody

Human IgG Polyclonal Antibody for WB, IHC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Therapeutic strategies targeting uPAR potentiate anti-PD-1 efficacy in diffuse-type gastric cancer.

LncRNA-H19 induces hepatic stellate cell activation via upregulating alcohol dehydrogenase III-mediated retinoic acid signals.

Fructose-derived glycation and immune function: Effects on antigen binding in human IgG and lymphocytes

Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes.

Compact Surface Plasmon Resonance IgG Sensor Based on H-Shaped Optical Fiber.

FAM64A promotes HNSCC tumorigenesis by mediating transcriptional autoregulation of FOXM1.