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Species Reactivity
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Human IgG Polyclonal antibody

Human IgG Polyclonal Antibody for WB, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10284

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated Human IgG Polyclonal (CL488-10284)

    WB analysis using CL488-10284

    Human plasma were subjected to SDS PAGE followed by western blot with CL488-10284 (Human IgG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated Human IgG Polyclonal (CL488-10284)

    IF Staining of human tonsillitis using CL488-10284

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 Human IgG antibody (CL488-10284) at dilution of 1:200.

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Tested Applications

Positive WB detected inhuman plasma
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL488-10284 targets Human IgG in WB, IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name Human IgG
Calculated molecular weight 155 kDa
Observed molecular weight 25 kDa, 50 kDa, 150 kDa
Gene symbol
Gene ID (NCBI)
RRIDAB_2918970
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This rabbit poly clonal antibody recognizes the human IgG.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 Human IgG antibody CL488-10284Download protocol
IF protocol for CL Plus 488 Human IgG antibody CL488-10284Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Oxid Med Cell Longev

Adropin Improves Radiation-Induced Myocardial Injury via VEGFR2/PI3K/Akt Pathway

Authors - Bingda Li
View Article