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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    WB analysis using 66045-1-Ig (same clone as 66045-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    WB analysis of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    WB analysis of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    IHC staining of human pancreas cancer using 66045-1-Ig (same clone as 66045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    IHC staining of human pancreas cancer using 66045-1-Ig (same clone as 66045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    IHC staining of human brain using 66045-1-Ig (same clone as 66045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IA-2/PTPRN Monoclonal antibody (66045-1-Ig)

    IF Staining of HepG2 using 66045-1-Ig (same clone as 66045-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66045-1-PBS in a different storage buffer formulation.

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IA-2/PTPRN Monoclonal antibody, PBS Only

IA-2/PTPRN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66045-1-PBS
Clone No.1D3F5

Host / Isotype

Mouse / IgG3

Reactivity

human, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

IA-2, PTPRN, 1D3F5, ICA512-transmembrane fragment, ICA512-N-terminal fragment

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66045-1-PBS targets IA-2/PTPRN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig, rabbit samples.

Tested Reactivity human, pig, rabbit
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen IA-2/PTPRN fusion protein Ag17909 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, N
Calculated molecular weight 105 kDa
Observed molecular weight 60-65 kDa, 106-115 kDa
GenBank accession numberBC007713
Gene Symbol IA-2/PTPRN
Gene ID (NCBI) 5798
RRIDAB_11064603
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16849
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.