Validation Data Gallery
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Tested Applications
| Positive WB detected in | pig brain tissue, human brain tissue, rabbit brain tissue |
| Positive IHC detected in | human pancreas cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66045-1-Ig targets IA-2/PTPRN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig, rabbit samples.
| Tested Reactivity | human, pig, rabbit |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | IA-2/PTPRN fusion protein Ag17909 相同性解析による交差性が予測される生物種 |
| Full Name | protein tyrosine phosphatase, receptor type, N |
| Calculated molecular weight | 105 kDa |
| Observed molecular weight | 60-65 kDa, 106-115 kDa |
| GenBank accession number | BC007713 |
| Gene Symbol | IA-2/PTPRN |
| Gene ID (NCBI) | 5798 |
| RRID | AB_11064603 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16849 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IA-2/PTPRN antibody 66045-1-Ig | Download protocol |
| IHC protocol for IA-2/PTPRN antibody 66045-1-Ig | Download protocol |
| IF protocol for IA-2/PTPRN antibody 66045-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |