IBA1 Recombinant antibody, PBS Only

Name: IBA1 Recombinant antibody, PBS Only
Brand: Proteintech
SKU: 81728-1-PBS

IBA1 Recombinant Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D5

Review Product

Cat no : 81728-1-PBS

Synonyms

AIF1, G1, allograft inflammatory factor 1, AIF-1, AIF 1

Validation Data Gallery

Filter:

AllWBIHCIF-PIF-FroFC (Intra)

WB analysis of THP-1 using 81728-1-RR (same clone as 81728-1-PBS)
THP-1 cells were subjected to SDS PAGE followed by western blot with 81728-1-RR (IBA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
WB analysis using 81728-1-RR (same clone as 81728-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 81728-1-RR (IBA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 81728-1-RR (same clone as 81728-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR006). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR006). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IF Staining of adult mouse cerebellum tissue using 81728-1-RR (same clone as 81728-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed adult mouse cerebellum tissue using IBA1 antibody (81728-1-RR) at dilution of 1:500 and Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L). Heat mediated antigen retrieval with PBS buffer (pH 7.4). (Image produced by Watt Lab at McGill University) This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed adult mouse cerebellum tissue using IBA1 antibody (81728-1-RR) at dilution of 1:500 and Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L). Heat mediated antigen retrieval with PBS buffer (pH 7.4). (Image produced by Watt Lab at McGill University) This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 81728-1-RR (same clone as 81728-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
FC experiment of THP-1 using 81728-1-RR (same clone as 81728-1-PBS)
1x10^6 THP-1 cells were intracellularly stained with 0.4 ug IBA1 Recombinant antibody (81728-1-RR, Clone:4D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.
×
1x10^6 THP-1 cells were intracellularly stained with 0.4 ug IBA1 Recombinant antibody (81728-1-RR, Clone:4D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

-/ -

Cat No. 81728-1-PBS

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81728-1-PBS targets IBA1 in WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	IBA1 fusion protein Ag1363 相同性解析による交差性が予測される生物種
Full Name	allograft inflammatory factor 1
Calculated molecular weight	17 kDa
Observed molecular weight	17 kDa
GenBank accession number	BC009474
Gene symbol	IBA1
Gene ID (NCBI)	199
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IBA1 Recombinant antibody, PBS Only

IBA1 Recombinant Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Validation Data Gallery

WB analysis of THP-1 using 81728-1-RR (same clone as 81728-1-PBS)

WB analysis using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of mouse liver using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of mouse brain using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of mouse brain using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of human liver using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of human liver using 81728-1-RR (same clone as 81728-1-PBS)

IHC staining of mouse liver using 81728-1-RR (same clone as 81728-1-PBS)

IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

IF Staining of adult mouse cerebellum tissue using 81728-1-RR (same clone as 81728-1-PBS)

IF Staining of mouse brain using 81728-1-RR (same clone as 81728-1-PBS)

FC experiment of THP-1 using 81728-1-RR (same clone as 81728-1-PBS)

Tested Applications

Recommended dilution

Product Information

Background Information