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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IBA1 Recombinant antibody, PBS Only

IBA1 Recombinant Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D5

Cat no : 81728-1-PBS

Synonyms

AIF1, G1, allograft inflammatory factor 1, AIF-1, AIF 1

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using IBA1 Recombinant antibody (81728-1-RR)

    WB analysis of THP-1 using 81728-1-RR (same clone as 81728-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 81728-1-RR (IBA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IBA1 Recombinant antibody (81728-1-RR)

    WB analysis using 81728-1-RR (same clone as 81728-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81728-1-RR (IBA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using IBA1 Recombinant antibody (81728-1-RR)

    IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using IBA1 Recombinant antibody (81728-1-RR)

    IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using IBA1 Recombinant antibody (81728-1-RR)

    IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using IBA1 Recombinant antibody (81728-1-RR)

    IHC staining of mouse liver using 81728-1-RR (same clone as 81728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using IBA1 Recombinant antibody (81728-1-RR)

    IHC staining of mouse liver using 81728-1-RR (same clone as 81728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using IBA1 Recombinant antibody (81728-1-RR)

    IHC staining of human liver using 81728-1-RR (same clone as 81728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using IBA1 Recombinant antibody (81728-1-RR)

    IHC staining of human liver using 81728-1-RR (same clone as 81728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using IBA1 Recombinant antibody (81728-1-RR)

    IHC staining of mouse brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using IBA1 Recombinant antibody (81728-1-RR)

    IHC staining of mouse brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using IBA1 Recombinant antibody (81728-1-RR)

    IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using IBA1 Recombinant antibody (81728-1-RR)

    IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using IBA1 Recombinant antibody (81728-1-RR)

    IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR006). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using IBA1 Recombinant antibody (81728-1-RR)

    IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of adult mouse cerebellum tissue using IBA1 Recombinant antibody (81728-1-RR)

    IF Staining of adult mouse cerebellum tissue using 81728-1-RR (same clone as 81728-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed adult mouse cerebellum tissue using IBA1 antibody (81728-1-RR) at dilution of 1:500 and Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L). Heat mediated antigen retrieval with PBS buffer (pH 7.4). (Image produced by Watt Lab at McGill University) This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using IBA1 Recombinant antibody (81728-1-RR)

    IF Staining of mouse brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of THP-1 cells using IBA1 Recombinant antibody (81728-1-RR)

    FC experiment of THP-1 using 81728-1-RR (same clone as 81728-1-PBS)

    1x10^6 THP-1 cells were intracellularly stained with 0.4 ug IBA1 Recombinant antibody (81728-1-RR, Clone:4D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81728-1-PBS targets IBA1 in WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IBA1 fusion protein Ag1363 相同性解析による交差性が予測される生物種
Full Name allograft inflammatory factor 1
Calculated molecular weight 17 kDa
Observed molecular weight17 kDa
GenBank accession numberBC009474
Gene symbol IBA1
Gene ID (NCBI) 199
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.