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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ICAM2/CD102 Monoclonal antibody

ICAM2/CD102 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H3C6

Cat no : 67628-1-Ig

Synonyms

CD102, ICAM2, ICAM-2, ICAM 2, 1H3C6

Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using ICAM2 Monoclonal antibody (67628-1-Ig)

    WB analysis of human peripheral blood platelets using 67628-1-Ig

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67628-1-Ig (ICAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ICAM2 Monoclonal antibody (67628-1-Ig)

    WB analysis of Jurkat using 67628-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67628-1-Ig (ICAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using ICAM2/CD102 Monoclonal antibody (67628-1-Ig)

    IF Staining of HUVEC using 67628-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using ICAM2/CD102 antibody (67628-1-Ig, Clone: 1H3C6 ) at dilution of 1:1991 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

Positive WB detected inhuman peripheral blood platelets, Jurkat cells
Positive IF/ICC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:995-1:3982
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67628-1-Ig targets ICAM2/CD102 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ICAM2/CD102 fusion protein Ag29441 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 2
Calculated molecular weight 31 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC003097
Gene symbol ICAM2/CD102
Gene ID (NCBI) 3384
RRIDAB_2882829
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.

Protocols

Product Specific Protocols
WB protocol for ICAM2/CD102 antibody 67628-1-IgDownload protocol
IF protocol for ICAM2/CD102 antibody 67628-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols