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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ICAM2 Monoclonal antibody, PBS Only

ICAM2 Monoclonal Antibody for WB, FC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H3C6

Cat no : 67628-1-PBS

Synonyms

CD102, ICAM 2, ICAM2

Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using ICAM2 Monoclonal antibody (67628-1-Ig)

    WB analysis of human peripheral blood platelets using 67628-1-Ig (same clone as 67628-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67628-1-Ig (ICAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67628-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ICAM2 Monoclonal antibody (67628-1-Ig)

    WB analysis of Jurkat using 67628-1-Ig (same clone as 67628-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67628-1-Ig (ICAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67628-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HUVEC cells using ICAM2 Monoclonal antibody (67628-1-Ig)

    FC experiment of HUVEC using 67628-1-Ig (same clone as 67628-1-PBS)

    1X10^6 HUVEC cells were stained with 0.2 ug Anti-Human ICAM2 (67628-1-Ig, Clone:1H3C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug mouse IgG2a isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 67628-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67628-1-PBS targets ICAM2 in WB, FC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ICAM2 fusion protein Ag29441 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 2
Calculated molecular weight 31 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC003097
Gene symbol ICAM2
Gene ID (NCBI) 3384
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.