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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 750-conjugated IDH1 Recombinant antibody

IDH1 Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

6L18

Cat no : CL750-81176

Synonyms

IDH 1, IDH, IDCD, EC:1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 750-conjugated IDH1 Recombinant ant (CL750-81176)

    WB analysis using CL750-81176

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-81176 (IDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 750-conjugated IDH1 Recombinant ant (CL750-81176)

    FC experiment of HepG2 using CL750-81176

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated IDH1 Recombinant antibody (CL750-81176, Clone:6L18)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue, LNCaP cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-81176 targets IDH1 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IDH1 fusion protein Ag19749 相同性解析による交差性が予測される生物種
Full Name isocitrate dehydrogenase 1 (NADP+), soluble
Calculated molecular weight 414 aa, 47 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC012846
Gene symbol IDH1
Gene ID (NCBI) 3417
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171). IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 IDH1 antibody CL750-81176Download protocol
FC protocol for CL Plus 750 IDH1 antibody CL750-81176Download protocol
Standard Protocols
Click here to view our Standard Protocols