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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated IDH2 Monoclonal antibody

IDH2 Monoclonal Antibody for WB, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F10A10

Cat no : CL488-66918

Synonyms

IDHM, IDH 2, ICD-M, ICD M, EC:1.1.1.42

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated IDH2 Monoclonal anti (CL488-66918)

    WB analysis using CL488-66918

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66918 (IDH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated IDH2 Monoclonal anti (CL488-66918)

    FC experiment of HepG2 using CL488-66918

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated IDH2 Monoclonal antibody (CL488-66918, Clone:1F10A10)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHEK-293 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66918 targets IDH2 in WB, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IDH2 fusion protein Ag8950 相同性解析による交差性が予測される生物種
Full Name isocitrate dehydrogenase 2 (NADP+), mitochondrial
Calculated molecular weight 452 aa, 51 kDa
Observed molecular weight 41-47 kDa
GenBank accession numberBC009244
Gene symbol IDH2
Gene ID (NCBI) 3418
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 IDH2 antibody CL488-66918Download protocol
Standard Protocols
Click here to view our Standard Protocols