CoraLite® Plus 488-conjugated IDO1 Monoclonal antibody
IDO1 Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
WB
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3G2G11
Cat no : CL488-66528
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | IFN gamma treated HeLa cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66528 targets IDO1 in WB applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | IDO1 fusion protein Ag3953 相同性解析による交差性が予測される生物種 |
Full Name | indoleamine 2,3-dioxygenase 1 |
Calculated molecular weight | 403 aa, 45 kDa |
Observed molecular weight | 42 kDa |
GenBank accession number | BC027882 |
Gene symbol | IDO1 |
Gene ID (NCBI) | 3620 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).
Protocols
Product Specific Protocols | |
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WB protocol for CL Plus 488 IDO1 antibody CL488-66528 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |