Iduronate 2 sulfatase Monoclonal antibody
Iduronate 2 sulfatase Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1A3F9
Cat no : 66112-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Human placenta, rat brain tissue, human brain tissue, mouse brain tissue, pig brain tissue |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66112-1-Ig targets Iduronate 2 sulfatase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Iduronate 2 sulfatase fusion protein Ag19095 相同性解析による交差性が予測される生物種 |
Full Name | iduronate 2-sulfatase |
Calculated molecular weight | 312aa,35 kDa; 550aa,62 kDa |
Observed molecular weight | 76 kDa, 55 kDa |
GenBank accession number | BC006170 |
Gene symbol | IDS |
Gene ID (NCBI) | 3423 |
RRID | AB_2881511 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).
Protocols
Product Specific Protocols | |
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WB protocol for Iduronate 2 sulfatase antibody 66112-1-Ig | Download protocol |
IHC protocol for Iduronate 2 sulfatase antibody 66112-1-Ig | Download protocol |
IF protocol for Iduronate 2 sulfatase antibody 66112-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |